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Anti-Inflammatory Action GHK-Cu downregulates TNF- and other pro-inflammatory cytokines in the scalp that contribute to follicle miniaturization and premature shedding

4.5.2 ER stress-induced CHAC1 expression and ferroptosis ER stress-induced CHAC1 expression contributes to ferroptosis by disrupting GSH homeostasis, which is crucial for cellular antioxidant defense

SDSPAGE and western blotting Samples were fractioned by SDSPAGE on 8% gels under reducing conditions, immunoblotted onto nitrocellulose membranes (GE Healthcare) and bands detected by enhanced chemiluminescence (GE Healthcare) onto films developed on a Xograph Compact X5 processor

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This approach resembles natural pancreatic development by guiding cells through stages like definitive endoderm, gut-tube endoderm, pancreatic endoderm, and endocrine precursors